Direct Visualization of Abasic Sites on a Single DNA Molecule Using Fluorescence Microscopy¶

Tamaki Hirose; Toshio Ohtani; Hiroshi Muramatsu; Atsushi Tanaka

doi:10.1562/0031-8655(2002)076<0123:DVOASO>2.0.CO;2

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1 August 2002 Direct Visualization of Abasic Sites on a Single DNA Molecule Using Fluorescence Microscopy

Tamaki Hirose, Toshio Ohtani, Hiroshi Muramatsu, Atsushi Tanaka

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Photochemistry and Photobiology, 76(2):123-126 (2002). https://doi.org/10.1562/0031-8655(2002)076<0123:DVOASO>2.0.CO;2

Abstract

A new method was developed to allow direct visualization of damaged sites on individual DNA molecules. Fluorescence in situ hybridization on extended DNA molecules was modified to detect a single abasic site. Abasic sites were specifically labeled with a biotinylated aldehyde-reactive probe and fluorochrome-conjugated streptavidin. The light emitted by a single fluorochrome–DNA complex was calibrated. The number of abasic sites on the DNA molecule was estimated by counting each fluorochrome–DNA complex. The present study directly visualized and characterized the abasic sites of single DNA molecules.

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Tamaki Hirose, Toshio Ohtani, Hiroshi Muramatsu, and Atsushi Tanaka "Direct Visualization of Abasic Sites on a Single DNA Molecule Using Fluorescence Microscopy," Photochemistry and Photobiology 76(2), 123-126, (1 August 2002). https://doi.org/10.1562/0031-8655(2002)076<0123:DVOASO>2.0.CO;2

Received: 16 November 2001; Accepted: 1 May 2002; Published: 1 August 2002

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